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43-163 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-163 (2ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-163 (2ug/ml) staining of Pig Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-163 (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PRMT2 Antibody

CATALOG NUMBER: 43-163

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-DQPRTTKYHSVILQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in lysates of cell line HeLa and in rodent Brain lysates (calculated MW of 37.2kDa according to NP_001229793.1). Approx 35kDa band observed in Pig Brain lysates (calculated MW of 38.7kDa according to XP_005658963.1).Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_001526.2; NP_001229793.1; NP_001229794.1; NP_001229795.1). Reported variants represent identical protein: NP_001526.2, NP_996845.1
Predicted Molecular Weight:
Approx 37kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PRMT2
Additional Names:
PRMT2, protein arginine methyltransferase 2, HRMT1L1, MGC111373, HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1, HMT1 hnRNP methyltransferase-like 1, OTTHUMP00000046525, OTTHUMP00000046526, OTTHUMP00000115881, OTTHUMP00000115882, OTTHUMP00000115884, OTTHUMP00000207831, PRMT2 alpha, PRMT2 beta, PRMT2 gamma, histone-arginine N-methyltransferase PRMT2, protein arginine N-methyltransferase 2
Protein Accession Number:
NP_001526.2,NP_001229793.1,NP_001229794.1,NP_001229795.1
Protein Gi Number:
46255047
Ncbi Gene Id Number:
3275
Background Reference 1:
Pak ML, Lakowski TM, Thomas D, Vhuiyan MI, Hüsecken K, Frankel A.A protein arginine N-methyltransferase 1 (PRMT1) and 2 heteromeric interaction increases PRMT1 enzymatic activity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-163

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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