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Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat 2.5 ug/ml.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.

PRMT5 Antibody

CATALOG NUMBER: 29-204

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PRMT5 antibody can be used for detection of PRMT5 by ELISA at 1:62500. PRMT5 antibody can be used for detection of PRMT5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
68 kDa, 73 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PRMT5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PRMT5
Additional Names:
PRMT5, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5
Protein Accession Number:
NP_001034708
Protein Gi Number:
88900507
Ncbi Gene Id Number:
10419
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription.
Background Reference 1:
Azzouz, T.N., (2005) J. Biol. Chem. 280 (41), 34435-34440.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-204

List Size:
100 ul

List Price:

$473.00

Datasheet
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