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Western blot analysis of anti- in HL60 cell line lysates (35ug/lane).
Western blot analysis of anti- in HL60 cell line lysates (35ug/lane).

PRMT5 Antibody

CATALOG NUMBER: 55-030

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Xenopus
Immunogen:
This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-499 amino acids from the C-terminal region of human PRMT5.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
73 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRMT5
Additional Names:
Protein arginine N-methyltransferase 5, 211-, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, SKB1 homolog, SKB1Hs, Protein arginine N-methyltransferase 5, N-terminally processed, PRMT5, HRMT1L5, IBP72, JBP1, SKB1
Protein Accession Number:
O14744
Ncbi Gene Id Number:
10419
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Background Reference 1:
Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003).
Background Reference 2:
Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001).
Background Reference 3:
Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999).
Background Reference 4:
Gilbreth, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95(25):14781-14786 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-030

List Size:
400 ul

List Price:

$495.00

Datasheet
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