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Western blot analysis of extracts of various cell lines, using PROCR antibody (15-122) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using PROCR antibody (15-122) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PROCR antibody (15-122) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using PROCR antibody (15-122) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PROCR Antibody

CATALOG NUMBER: 15-122

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-209 of human PROCR (NP_006395.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
NCI-H460
Positive Control 2:
HT-29
Positive Control 3:
SKOV3
Positive Control 4:
A549
Positive Control 5:
THP-1
Predicted Molecular Weight:
Observed: 50kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PROCR
Additional Names:
Endothelial protein C receptor, Activated protein C receptor, APC receptor, Endothelial cell protein C receptor, CD201, PROCR, EPCR
Ncbi Gene Id Number:
10544
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-122

List Size:
100 uL

List Price:

$436.00

Datasheet
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