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46-227 (0.3ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-227 (0.3ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, Cy3-staining. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
46-227 (0.3ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Proenkephalin Antibody

CATALOG NUMBER: 46-227

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RSHHQDGSDNEE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology. 1998 Apr;139(4):2137-45; PMID: 9529003). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration: 0.3-1ug/ml.
Positive Control 1:
Cat. No. XBL-11050 - Human Adrenal Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PENK
Additional Names:
proenkephalin, PENK
Protein Accession Number:
NP_006202.1
Protein Gi Number:
5453876
Ncbi Gene Id Number:
5179
Background Reference 1:
Schiltz CA, Bremer QZ, Landry CF, Kelley AE. Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression. BMC Biol. 2007 Apr 26;5:16.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-227

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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