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42-217 (1ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-217 (0.1ug/ml) staining of Human Cerebellum (A), Rat Brain (B) and Rat Testis (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-217 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-217 (1ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PROKR2 Antibody

CATALOG NUMBER: 42-217

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Immunogen:
The immunogen for this antibody is: C-NGNTSFTPNFN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum), Testis and Thyroid Gland and in Rat Brain, Stomach and Testis lysates (calculated MW of 44.0kDa according to Human NP_658986.1 and 47.9kDa according to Rat NP_620434.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1475 - Rat Stomach Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PROKR2
Additional Names:
PROKR2, prokineticin receptor 2, GPR73L1, GPR73b, GPRg2, KAL3, PKR2, dJ680N4.3, G protein-coupled receptor 73-like 1, OTTHUMP00000030192
Protein Accession Number:
NP_658986.1
Protein Gi Number:
21426829
Ncbi Gene Id Number:
128674
Background Reference 1:
Monnier C, Dode C, Fabre L, Teixeira L, Labesse G, Pin JP, Hardelin JP, Rondard P. PROKR2 missense mutations associated with Kallmann syndrome impair receptor signalling activity. Human molecular genetics 2009 Jan 18 (1): 75-81.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-217

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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