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Western blot analysis of PROM1 in Jurkat cell lysate with PROM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of PROM1 in Jurkat cells with PROM1 antibody at 2.5 μg/mL.
Immunofluorescence of PROM1 in Jurkat cells with PROM1 antibody at 20 μg/mL.
Western blot analysis of PROM1 in Jurkat cell lysate with PROM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

PROM1 Antibody

CATALOG NUMBER: 7783

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PROM1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PROM1.
The immunogen is located within the last 50 amino acids of PROM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PROM1 antibody can be used for detection of PROM1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PROM1 antibody is human, mouse and rat reactive. Multiple isoforms of PROM1 are known to exist.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Predicted Molecular Weight:
Predicted: 95 kDa
Observed: 97 kDa
Purification:
PROM1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PROM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PROM1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PROM1
Additional Names:
PROM1 Antibody: RP41, AC133, CD133, MCDR2, STGD4, CORD12, PROML1, MSTP061, Prominin-1, Antigen AC133
Protein Accession Number:
NP_006008
Protein Gi Number:
5174387
Ncbi Gene Id Number:
8842
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PROM1 is a pentaspan transmembrane glycoprotein that localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation (1). Mutations in this gene have been shown to result in retinitis pigmentosa (2). Expression of this gene is also associated with several types of cancer (3).
Background Reference 1:
Yin AH, Miraglia S, Zanjani ED, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 1997; 90:5002-12.
Background Reference 2:
Zhang Q, Zulfiqar F, Xiao X, et al. Severe retinitis pigmentosa mapped to 4p15 and associated with a novel mutation in the PROM1 gene. Hum. Genet. 2007; 122:293-9.
Background Reference 3:
Tabu K, Bizen N, Taga T, et al. Gene regulation of Prominin-1 (CD133) in normal and cancerous tissues. Adv. Exp. Med. Biol. 2013; 777:73-85.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7783

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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