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Recombinant Protein G, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
Recombinant Protein G, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Protein G Recombinant Protein

CATALOG NUMBER: 96-655

Tested Applications:
WB
Specifications
source-species:
E. coli
Species:
Others
source-species:
E. coli
Recombinant Protein Sequence:
Asp23 - Arg227 (beta chain) & Ser228 - Glu449 (alpha chain)
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Purity:
>98% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
0.1 M NaH2PO4, pH8.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Names:
RPG, Protein G
Background:
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.
Background Reference 1:
Sjobring U, Bjorck L, Kastern W, et al., 1991, J Biol Chem 266 : 399–405.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-655

List Size:
2 mg

List Price:

$275.00

Datasheet
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