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Fluorescent image of U251 cell stained with PPP1CB Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Western blot analysis of PPP1CB using rabbit polyclonal PPP1CB Antibody (K301) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.
Fluorescent image of U251 cell stained with PPP1CB Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Protein Phosphatase 1 beta Antibody

CATALOG NUMBER: 63-467

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus
Immunogen:
This Protein Phosphatase 1 beta (PPP1CB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human Protein Phosphatase 1 beta (PPP1CB).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPP1CB
Additional Names:
Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB
Protein Accession Number:
P62140
Protein Gi Number:
49065814
Ncbi Gene Id Number:
5500
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein phosphatase-1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases involved in the dephosphorylation of a variety of proteins. These enzymes work in opposition to the protein kinases to control the level of phosphorylation. Protein phosphatase (PP1) is essential for cell division, and it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis, as well as in regulation of ionic conductances and long-term synaptic plasticity. PP1 has 3 catalytic subunits, designated alpha (PPP1CA), beta (PPP1CB), and gamma (PPP1CC).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-467

List Size:
400 ul

List Price:

$495.00

Datasheet
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