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Protocadherin X Antibody

CATALOG NUMBER: 42-252

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-KYNWVTTPTTFK
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 75kDa band in Human Brain (Amygdala, Hippocampus, Substantia nigra) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kDa according to NP_116753.1 and of 113kDa according to NP_055337.1, the smallest reported isoforms. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize all reported Y-linked isoforms (NP_116753.1; NP_116754.1; NP_116755.1) and all reported X-linked isoforms (NP_055337.1; NP_055337.1; NP_116750.1; NP_116751.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PCDH11Y
Additional Names:
PCDH11Y, protocadherin 11 Y-linked, PCDH-PC, PCDH11X, PCDH22, PCDHY, OTTHUMP00000033077, protocadherin 11 X-linked, protocadherin 22, protocadherin Y, RP13-138P15.1, PCDH-X, PCDH11, PCDHX, protocadherin 11X, protocadherin X, protocadherin-S
Protein Accession Number:
NP_116753.1,NP_116754.1,NP_116755.1,NP_055337.1,NP_055337.1,NP_116750.1,NP_116751.1
Protein Gi Number:
14589942
Ncbi Gene Id Number:
83259;27328
Background Reference 1:
Kalmady SV, Venkatasubramanian G, Evidence for positive selection on Protocadherin Y gene in Homo sapiens: implications for schizophrenia. Schizophrenia research 2009 Mar 108 (1-3): 299-300.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-252

List Size:
0.1 mg

List Price:

$468.00

Datasheet
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