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Western blot analysis of anti-PRP4 Pab in Jurkat cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-PRP4 Pab in Jurkat cell lysate

PRP4 Antibody

CATALOG NUMBER: 62-982

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This PRP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 948-978 amino acids from the C-terminal region of human PRP4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
117 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRPF4B
Additional Names:
Serine/threonine-protein kinase PRP4 homolog, PRP4 kinase, PRP4 pre-mRNA-processing factor 4 homolog, PRPF4B, KIAA0536, PRP4, PRP4H, PRP4K
Protein Accession Number:
Q13523
Protein Gi Number:
317373526
Ncbi Gene Id Number:
8899
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Pre-mRNA splicing occurs in two sequential transesterification steps, and PRP4 is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).
Background Reference 1:
Dellaire, G., et al., Mol. Cell. Biol. 22(14):5141-5156 (2002).
Background Reference 2:
Kojima, T., et al., J. Biol. Chem. 276(34):32247-32256 (2001).
Background Reference 3:
Huang, Y., et al., Biochem. Biophys. Res. Commun. 271(2):456-463 (2000).
Background Reference 4:
Gross, T., et al., Nucleic Acids Res. 25(5):1028-1035 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-982

List Size:
400 ul

List Price:

$495.00

Datasheet
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