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Western blot analysis of extracts of various cell lines, using PRPF31 antibody (19-821) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRPF31 antibody (19-821) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PRPF31 antibody (19-821) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PRPF31 Antibody

CATALOG NUMBER: 19-821

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRPF31 (NP_056444.3).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
U-251MG
Positive Control 2:
Jurkat
Positive Control 3:
HepG2
Positive Control 4:
Mouse lung
Positive Control 5:
Mouse testis
Positive Control 6:
Rat lung
Predicted Molecular Weight:
Observed: 56kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PRPF31
Additional Names:
pre-mRNA processing factor 31 homolog, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), Protein 61K, pre-mRNA processing factor 31 homolog (yeast), DKFZp566J153, NY-BR-99, PRP31, RP11, PRP31 pre-mRNA processing factor 31 homolog (yeast), PRPF31
Ncbi Gene Id Number:
26121
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-821

List Size:
100 uL

List Price:

$436.00

Datasheet
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