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Western blot analysis of extracts of various cell lines, using PRPF8 antibody (19-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (19-988) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (19-988) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PRPF8 antibody (19-988) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

PRPF8 Antibody

CATALOG NUMBER: 19-988

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Predicted Molecular Weight:
Observed: 274kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PRPF8
Additional Names:
HPRP8, PRP8, PRPC8, RP13, SNRNP22pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p, apoptosis-regulated protein 1, apoptosis-regulated protein 2, p22precursor mRNA processing protein, splicing factor Prp8
Ncbi Gene Id Number:
10594
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-988

List Size:
100 uL

List Price:

$436.00

Datasheet
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