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Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunocytochemistry of PPR5L in 293 cells with PPR5L antibody at 10 μg/mL.
Immunofluorescence of PPR5L in3T3 cells with PPR5L antibody at 20 μg/mL.
Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

PRR5L Antibody

CATALOG NUMBER: 6293

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (88%)
Immunogen:
PRR5L antibody was raised against a 17 amino acid synthetic peptide near the center of human PRR5L.
The immunogen is located within amino acids 220 - 270 of PRR5L.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PRR5L antibody can be used for detection of PRR5L by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of PRR5L are known to exist; this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Positive Control 3:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
PRR5L Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PRR5L Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PRR5L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRR5L
Additional Names:
PRR5L Antibody: PROTOR2, PROTOR2, Proline-rich protein 5-like, Protein observed with Rictor-2, Protor-2
Protein Accession Number:
NP_001153639
Protein Gi Number:
237757351
Ncbi Gene Id Number:
79899
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRR5L Antibody: Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2). PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.
Background Reference 1:
Thedieck K, Polak P, Kim ML, et al. 2007. PRAS40 and PRR5-like protein are new mTOR interactors that regulate apoptosis. PLoS One 2007; 2:e1217.
Background Reference 2:
Pearce LR, Huang X, Boudeau J, et al. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. Biochem. J. 2007; 405:513-22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6293

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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