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Western blot analysis of extracts of various cell lines, using PRSS1 antibody (14-411) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human lung cancer using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PRSS1 antibody (14-411) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PRSS1 antibody (14-411) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PRSS1 Antibody

CATALOG NUMBER: 14-411

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-247 of human PRSS1 (NP_002760.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
Positive Control 1:
HT-29
Positive Control 2:
Mouse spleen
Positive Control 3:
Mouse kidney
Predicted Molecular Weight:
Observed: 35kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PRSS1
Additional Names:
Trypsin-1, Beta-trypsin, Cationic trypsinogen, Serine protease 1, Trypsin I, Alpha-trypsin chain 1, Alpha-trypsin chain 2, PRSS1, TRP1, TRY1, TRYP1
Ncbi Gene Id Number:
5644
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-411

List Size:
50 uL

List Price:

$309.00

Out of stock

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