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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
PRSS3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

PRSS3 Antibody

CATALOG NUMBER: 55-214

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PRSS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human PRSS3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Names:
Trypsin-3, Brain trypsinogen, Mesotrypsinogen, Serine protease 3, Serine protease 4, Trypsin III, Trypsin IV, PRSS3, PRSS4, TRY3, TRY4
Protein Accession Number:
P35030
Protein Gi Number:
209572698
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRSS3 encodes a trypsinogen, which is a member of thetrypsin family of serine proteases. This enzyme is expressed in thebrain and pancreas and is resistant to common trypsin inhibitors.It is active on peptide linkages involving the carboxyl group oflysine or arginine. PRSS3 is localized to the locus of T cellreceptor beta variable orphans on chromosome 9. Four transcriptvariants encoding different isoforms have been described for thisgene.
Background Reference 1:
Jiang, G., et al. Gut 59(11):1535-1544(2010)
Background Reference 2:
Nakanishi, J., et al. J. Invest. Dermatol. 130(4):944-952(2010)
Background Reference 3:
Salameh, M.A., et al. J. Biol. Chem. 285(3):1939-1949(2010)
Background Reference 4:
Rosendahl, J., et al. Pancreatology 10 (2-3), 243-249 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-214

List Size:
400 ul

List Price:

$495.00

Datasheet
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