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Western blot analysis of PRUNE Antibody in Jurkat cell line lysates (35ug/lane)
PRUNE Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PRUNE Antibody in Jurkat cell line lysates (35ug/lane)

PRUNE Antibody

CATALOG NUMBER: 64-092

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PRUNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-389 amino acids from the C-terminal region of human PRUNE.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRUNE
Additional Names:
Protein prune homolog, hPrune, Drosophila-related expressed sequence 17, DRES-17, DRES17, HTcD37, PRUNE
Protein Accession Number:
Q86TP1
Protein Gi Number:
229462737
Ncbi Gene Id Number:
58497
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. It plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
Background Reference 1:
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)
Background Reference 2:
Middelhaufe, S., et al. Biochem. J. 407(2):199-205(2007)
Background Reference 3:
Kobayashi, T., et al. Mol. Cell. Biol. 26(3):898-911(2006)
Background Reference 4:
Zollo, M., et al. Clin. Cancer Res. 11(1):199-205(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-092

List Size:
400 ul

List Price:

$495.00

Datasheet
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