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Western blot analysis using KLK3 monoclonal antibody against truncated KLK3 - His recombinant protein
Immunohistochemical analysis of paraffin - embedded human prostate carcinoma tissue showing cytoplasma localization using KLK3 antibody with DAB staining.
Western blot analysis using KLK3 monoclonal antibody against truncated KLK3 - His recombinant protein

PSA Antibody [5A11E9]

CATALOG NUMBER: 32-206

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Purified truncated recombinant KLK3-His (aa26-251) is expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
PSA monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLK3
Additional Names:
APS, Gamma-seminoprotein, PSA, APS, hK3, KLK2A1
Protein Accession Number:
P07288
Protein Gi Number:
130989
Ncbi Gene Id Number:
354
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Kallikrein-related peptidase 3.Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.
Background Reference 1:
Am J Surg Pathol. 2007 Sep;31(9):1351-5.
Background Reference 2:
Chem Biol Drug Des. 2007 Sep;70(3):261-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-206

List Size:
0.1 mL

List Price:

$432.00

Datasheet
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