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Western blot analysis of extracts of various cell lines, using PSAT1 antibody (15-383) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using PSAT1 Antibody (15-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PSAT1 Antibody (15-383) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PSAT1 antibody (15-383) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PSAT1 Antibody

CATALOG NUMBER: 15-383

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human PSAT1 (NP_478059.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
SKOV3
Positive Control 3:
Mouse brain
Positive Control 4:
Rat kidney
Positive Control 5:
Rat brain
Predicted Molecular Weight:
Observed: 40kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PSAT1
Additional Names:
PSAT1, EPIP, MGC1460, PSA, PSAT
Ncbi Gene Id Number:
29968
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-383

List Size:
100 uL

List Price:

$436.00

Datasheet
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