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Western blot analysis of anti-PSKH1 Antibody (N-term H70) in Hela cell line lysate and mouse kidney tissue lysate (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-PSKH1 Antibody (N-term H70) in Hela cell line lysate and mouse kidney tissue lysate (35ug/lane)

PSKH1 Antibody

CATALOG NUMBER: 62-708

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This PSKH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-91 amino acids from the N-terminal region of human PSKH1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PSKH1
Additional Names:
Serine/threonine-protein kinase H1, Protein serine kinase H1, PSK-H1, PSKH1
Protein Accession Number:
P11801
Protein Gi Number:
17379144
Ncbi Gene Id Number:
5681
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PSKH1 may be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing. PSKH1 is localized in the Brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm.
Background Reference 1:
Brede, G., et al., Exp. Cell Res. 291(2):299-312 (2003).
Background Reference 2:
Brede, G., et al., Nucleic Acids Res. 30(23):5301-5309 (2002).
Background Reference 3:
Brede, G., et al., Genomics 70(1):82-92 (2000).
Background Reference 4:
Amarzguioui, M., et al., Nucleic Acids Res. 28(21):4113-4124 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-708

List Size:
400 ul

List Price:

$495.00

Datasheet
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