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42-829 (1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-829 (0.3ug/ml) staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-829 (1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PSMA4 Antibody

CATALOG NUMBER: 42-829

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-SDANVLTNELR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 28kDa band observed in lysates of cell lines K562, NIH3T3 and in lysates of Human Tonsil, Human Peripheral Blood Mononucleocytes and in Mouse and Rat Spleen (calculated MW of 29.5kDa according to NP_002780.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_002780.1; NP_001096138.1). Reported variants represent identical protein: NP_002780.1, NP_001096137.1
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PSMA4
Additional Names:
PSMA4, proteasome (prosome, macropain) subunit, alpha type, 4, HC9, HsT17706, MGC111191, MGC12467, MGC24813, PSC9, macropain subunit C9, multicatalytic endopeptidase complex subunit C9, proteasome alpha 4 subunit, proteasome component C9, proteasome subunit HC9, proteasome subunit L
Protein Accession Number:
NP_002780.1,NP_001096138.1
Protein Gi Number:
4506185
Ncbi Gene Id Number:
5685

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-829

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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