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Western Blot at 1:1000 dilution Lane 1: Daudi whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution Lane 1: Daudi whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.

PSMB10 Antibody

CATALOG NUMBER: 56-511

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PSMB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the C-terminal region of human PSMB10.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PSMB10
Additional Names:
Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i, PSMB10, LMP10, MECL1
Protein Accession Number:
P40306
Protein Gi Number:
730376
Ncbi Gene Id Number:
5699
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The proteasome is a multicatalytic proteinase complex witha highly ordered ring-shaped 20S core structure. The core structureis composed of 4 rings of 28 non-identical subunits; 2 rings arecomposed of 7 alpha subunits and 2 rings are composed of 7 betasubunits. Proteasomes are distributed throughout eukaryotic cellsat a high concentration and cleave peptides in anATP/ubiquitin-dependent process in a non-lysosomal pathway. Anessential function of a modified proteasome, the immunoproteasome,is the processing of class I MHC peptides. This gene encodes amember of the proteasome B-type family, also known as the T1Bfamily, that is a 20S core beta subunit. Proteolytic processing isrequired to generate a mature subunit. Expression of this gene isinduced by gamma interferon, and this gene product replacescatalytic subunit 2 (proteasome beta 7 subunit) in theimmunoproteasome.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care (2010) In press :
Background Reference 2:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 3:
Moschonas, A., et al. Mol. Cell. Biol. 28(20):6208-6222(2008)
Background Reference 4:
Liu, Y., et al. DNA Seq. 18(4):257-264(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-511

List Size:
400 ul

List Price:

$495.00

Datasheet
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