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Antibody used in WB on Human 293T at 1 ug/ml.
Antibody used in WB on Human Jurkat 1 ug/ml.
Antibody used in WB on Human HepG2 at 1 ug/ml.
Antibody used in WB on Human HeLa cells at 0.2-1 ug/ml.
Antibody used in WB on Human 293T at 1 ug/ml.

PSMB2 Antibody

CATALOG NUMBER: 31-331

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PSMB2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PSMB2 antibody can be used for detection of PSMB2 by ELISA at 1:312500. PSMB2 antibody can be used for detection of PSMB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
23 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PSMB2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PSMB2
Additional Names:
PSMB2, HC7-I, MGC104215, MGC126885
Protein Accession Number:
NP_002785
Protein Gi Number:
4506195
Ncbi Gene Id Number:
5690
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB2 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.
Background Reference 1:
Gregory, S.G., (2006) Nature 441 (7091), 315-321 .

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-331

List Size:
100 ul

List Price:

$519.00

Datasheet
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