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Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PSMB9 Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PSMB9 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.

PSMB9 Antibody

CATALOG NUMBER: 63-515

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PSMB9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-219 amino acids from the C-terminal region of human PSMB9.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PSMB9
Additional Names:
Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein, PSMB9, LMP2, PSMB6i, RING12
Protein Accession Number:
P28065
Protein Gi Number:
417529
Ncbi Gene Id Number:
5698
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PSMB9 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Background Reference 1:
Honcharov,S.V., et.al., Fiziol Zh 55 (2), 3-10 (2009)
Background Reference 2:
Moschonas,A., et.al., Mol. Cell. Biol. 28 (20), 6208-6222 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-515

List Size:
400 ul

List Price:

$495.00

Datasheet
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