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Western blot analysis of PSMD11 Antibody in 293, Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PSMD11 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Western blot analysis of PSMD11 Antibody in 293, Jurkat cell line lysates (35ug/lane)

PSMD11 Antibody

CATALOG NUMBER: 61-518

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This PSMD11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-317 amino acids from the C-terminal region of human PSMD11.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PSMD11
Additional Names:
26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p445, PSMD11
Protein Accession Number:
O00231
Ncbi Gene Id Number:
5717
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The 26S proteasome (PSMD11) is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The protein is a non-ATPase subunit of the 19S regulator.
Background Reference 1:
Saito,A., Gene 203 (2), 241-250 (1997)
Background Reference 2:
Hoffman,L., FEBS Lett. 404 (2-3), 179-184 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-518

List Size:
400 ul

List Price:

$495.00

Datasheet
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