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Antibody used in WB on Human Raji cells at 0.2-1 ug/ml.
Antibody used in WB on Human Raji cells at 0.2-1 ug/ml.

PSMD4 Antibody

CATALOG NUMBER: 27-556

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PSMD4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PSMD4 antibody can be used for detection of PSMD4 by ELISA at 1:62500. PSMD4 antibody can be used for detection of PSMD4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Predicted Molecular Weight:
41 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PSMD4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PSMD4
Additional Names:
PSMD4, AF, ASF, S5A, AF-1, MCB1, Rpn10, pUB-R5
Protein Accession Number:
NP_002801
Protein Gi Number:
5292161
Ncbi Gene Id Number:
5710
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD4 encodes one of the non-ATPase subunits of the 19S regulator lid.
Background Reference 1:
Fujiwara, K., et al., (2004) J. Biol. Chem. 279 (6), 4760-4767.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-556

List Size:
100 ul

List Price:

$519.00

Datasheet
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