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46-238 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-238 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PSME1 Antibody

CATALOG NUMBER: 46-238

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KNFEKLKKPRGETK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Human Lymph Node, Spleen, Tonsil and Peripheral Blood Mononuceocyte lysates (calculated MW of 28.7kDa according to NP_006254.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_006254.1).
Positive Control 1:
Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PSME1
Additional Names:
PSME1, proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), IFI5111, MGC8628, PA28A, PA28alpha, REGalpha , 11S regulator complex alpha subunit, 29-kD MCP activator subunit, activator of multicatalytic protease subunit 1, interferon gamma up-regulated I-5111 protein, interferon-gamma IEF SSP 5111, interferon-gamma-inducible protein 5111, proteasome activator subunit 1, proteasome activator subunit-1
Protein Accession Number:
NP_006254.1
Protein Gi Number:
5453990
Ncbi Gene Id Number:
5720
Background Reference 1:
Brychcy M, Kuckelkorn U, Hausdorf G, Egerer K, Kloetzel PM, Burmester GR, Feist E. Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28. Arthritis Rheum. 2006 Jul;54(7):2175-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-238

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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