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46-239 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-239 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-239 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PSME2 Antibody

CATALOG NUMBER: 46-239

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-NLEKIVNPKGEEKP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 27kDa band observed in Human Spleen lysates (calculated MW of 27.4kDa according to NP_002809.2). Recommended concentration: 0.5-1ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PSME2
Additional Names:
PSME2, proteasome (prosome, macropain) activator subunit 2 (PA28 beta), PA28B, PA28beta, REGbeta , 11S regulator complex beta subunit, MCP activator, 31-kD subunit, activator of multicatalytic protease subunit 2, cell migration-inducing protein 22, proteasome activator 28-beta, proteasome activator hPA28 subunit beta, proteasome activator subunit 2
Protein Accession Number:
NP_002809.2
Protein Gi Number:
30410792
Ncbi Gene Id Number:
5721
Background Reference 1:
Brychcy M, Kuckelkorn U, Hausdorf G, Egerer K, Kloetzel PM, Burmester GR, Feist E. Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28. Arthritis Rheum. 2006 Jul;54(7):2175-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-239

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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