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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with PSME2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

PSME2 Antibody

CATALOG NUMBER: 62-406

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig
Immunogen:
This PSME2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the Central region of human PSME2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
27 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PSME2
Additional Names:
Proteasome activator complex subunit 2, 11S regulator complex subunit beta, REG-beta, Activator of multicatalytic protease subunit 2, Proteasome activator 28 subunit beta, PA28b, PA28beta, PSME2
Protein Accession Number:
Q9UL46
Protein Gi Number:
296453017
Ncbi Gene Id Number:
5721
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 is the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon.
Background Reference 1:
Conticello,S.G., Curr. Biol. 13 (22), 2009-2013 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-406

List Size:
400 ul

List Price:

$495.00

Datasheet
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