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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human S100A7 with C-terminal MYC tag probed with 46-241 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 46-241 (1mg/ml) in Lane B. Prima
46-241 (0.1ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-241 (3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human S100A7 with C-terminal MYC tag probed with 46-241 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 46-241 (1mg/ml) in Lane B. Prima

Psoriasin Antibody

CATALOG NUMBER: 46-241

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HKYTRRDDKIDKP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 15kDa band observed in Human Skin lysates (calculated MW of 11.5kDa according to NP_002954.1). Recommended concentration: 1-3ug/ml.In transfected HEK293 transiently expressing full-length Human S100A7 (myc and DYKDDDDK tagged), a band of approx. 16kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5ug/ml.Approx 15kDa band was also observed in Human Skin lysates (calculated MW of 11.5kDa according to NP_002954.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 3.75ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
S100A7
Additional Names:
S100A7, S100 calcium binding protein A7 (psoriasin 1) , HGNC:10497, PSOR1, S100A7c , S100 calcium-binding protein A7, S100 calcium-binding protein A7 (psoriasin 1), psoriasin 1, Psoriasin
Protein Accession Number:
NP_002954.1
Protein Gi Number:
4506769
Ncbi Gene Id Number:
6278
Background Reference 1:
Webb M, Emberley ED, Lizardo M, Alowami S, Qing G, Alfia'ar A, Snell-Curtis LJ, Niu Y, Civetta A, Myal Y, Shiu R, Murphy LC, Watson PH. Expression analysis of the mouse S100A7/psoriasin gene in skin inflammation and mammary tumorigenesis. BMC Cancer. 2005 Feb 17;5(1):17.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-241

List Size:
0.1 mg

List Price:

$469.00

Out of stock

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