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46-243 (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-243 (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
46-243 (0.01ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-243 (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-243 (0.1-0.3ug/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-243 (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PTB Antibody

CATALOG NUMBER: 46-243

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DGIVPDIAVGTKR-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human, Mouse and Rat Ovary lysates and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat, HEK293 and 3T3N (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.01-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver and Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration: 2-4ug/ml.
Specificity:
This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PTBP1
Additional Names:
PTBP1, PTB, polypyrimidine tract binding protein 1, PTB2, PTB3, PTB4, pPTB, HNRPI, PTB-1, HNRNPI, RNA binding protein, heterogeneous nuclear ribonucleoprotein polypeptide I, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I), HNRNP-I, MGC10830, MGC8461, RNA-binding protein, heterogeneous nuclear ribonucleoprotein I
Protein Accession Number:
NP_002810.1,NP_114367.1,NP_114368.1,NP_787041.1
Protein Gi Number:
4506243
Ncbi Gene Id Number:
19205(mouse);29497(rat);
Background Reference 1:
Charlet-B N, Logan P, Singh G, Cooper TA. Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. Mol Cell. 2002 Mar;9(3):649-58.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-243

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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