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46-245 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-245 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (bl
46-245 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/membrane staining. The nuclear stain is DAP
46-245 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PTCH Antibody

CATALOG NUMBER: 46-245

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: ELQDVECEERPR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Human Colon shows textured nuclear staining of the absorptive cells. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml. This produt has been successfully used in IHC on Human: Abiria SA et al, J Neurooncol. 2014 May 28. PMID: 24867209.Immunofluorescence: Expression of the protein seen in the cytoplasm of HeLa and in the Golgi/membranes of NIH3T3 cells. Recommended concentration: 5ug/ml. This produt has been successfully used in IF on Human: Abiria SA et al, J Neurooncol. 2014 May 28. PMID: 24867209.
Specificity:
This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PTCH1
Additional Names:
PTCH1, PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b, PTCH protein +4', PTCH protein -10
Protein Accession Number:
NP_000255.1,NP_001077072.1,NP_001077071.1,NP_001077073.1,NP_001077074.1,NP_001077075.1,NP_001077076.1
Protein Gi Number:
4506247
Ncbi Gene Id Number:
5727
Background Reference 1:
Nagao K, Togawa N, Fujii K, Uchikawa H, Kohno Y, Yamada M, Miyashita T. Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays. Hum Mol Genet. 2005 Oct 3;

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-245

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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