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46-246 (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-246 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/membrane staining. The nuclear stain is DAP
46-246 (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PTCH Antibody

CATALOG NUMBER: 46-246

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HPESRHHPPSNPRQQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 153.9kDa according to NP_001077071.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PTCH
Additional Names:
PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1 , PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog
Protein Accession Number:
NP_000255.2,NP_001077072.1,NP_001077071.1,NP_001077075.1
Protein Gi Number:
4506247
Ncbi Gene Id Number:
5727
Background Reference 1:
Nagao K, Togawa N, Fujii K, Uchikawa H, Kohno Y, Yamada M, Miyashita T. Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays. Hum Mol Genet. 2005 Nov 15;14(22):3379-88. Epub 2005 Oct 3.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-246

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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