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Western blot analysis of PTEN in human brain tissue lysate with PTEN antibody at (A) 1 and (B) 2 μg/mL..
Immunofluorescence of PTEN in P815 cells with PTEN antibody at 2 μg/mL.
Western blot analysis of PTEN in human brain tissue lysate with PTEN antibody at (A) 1 and (B) 2 μg/mL..
Trial Size

PTEN Antibody

CATALOG NUMBER: 3517

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
PTEN antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PTEN.
The immunogen is located within the first 50 amino acids of PTEN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IP, WB
Application Note:
PTEN antibody can be used for the detection of PTEN by Western blot at 1 to 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1286 - P815 Cell Lysate
Positive Control 2:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 3:
Cat. No. 17-206 - P815 Cell Slide
Predicted Molecular Weight:
Predicted: 44, 63 kDa
Observed: 46 kDa
Purification:
PTEN Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PTEN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PTEN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTEN
Additional Names:
PTEN Antibody: BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, Phosphatidylinositol 3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1
Protein Accession Number:
P60484
Protein Gi Number:
42560209
Ncbi Gene Id Number:
5728
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PTEN Antibody: PTEN (phosphatase and tensin homologue deleted on chromosome ten) is a dual-specificity phosphatase (with both protein and lipid phosphatase activity) first identified as a tumor suppressor gene. PTEN indirectly activates the AKT/PI3K pathway, an important signaling pathway for cell growth and proliferation by keeping levels of the second messenger PIP3 low, thereby preventing phosphoinositide-dependent kinase-1 (PDK-1) from phosphorylating and activating AKT. Expression of wild-type PTEN causes growth arrest in many cancer cell lines, but expression of a PTEN protein containing a mutation that blocks its lipid phosphatase activity failed to suppress growth of glioma cell lines suggesting that the tumor suppressive effect of PTEN is mediated solely by its lipid phosphatase activity. Other activities include the inhibition of insulin stimulated MAPK activation by blocking the insulin-receptor substrate (IRS)-1 phosphorylation and assembly of the IRS-1/Grb2/Sos complex.
Background Reference 1:
Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science1997; 275:1943-7.
Background Reference 2:
Steck PA, Pershouse MA, Jasser SA, et al. Identification of a candidate tumor suppressor gene, MMAC1, a chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat. Genet.1997; 15:356-62.
Background Reference 3:
Wu X, Senechal K, Neshat MS, et al. The PTEN/MMAC1 tumor suppressor phosphatase functions as a negative regulator of the phosphoinositide 3-kinase/Akt pathway. Proc. Natl. Acad. Sci. USA1998; 95:15587-91.
Background Reference 4:
Myers MP, Pass I, Batty IH, et al. The lipid phosphatase activity of PTEN is critical for its tumor suppressor function. Proc. Natl. Acad. Sci. USA1998; 95:13513-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3517

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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