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Western blot analysis of extracts of various cell lines, using PTGES2 antibody (14-991) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of A549 cells using PTGES2 antibody (14-991). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PTGES2 antibody (14-991) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

PTGES2 Antibody

CATALOG NUMBER: 14-991

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 88-377 of human PTGES2 (NP_079348.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
HeLa
Positive Control 3:
BT-474
Positive Control 4:
HL-60
Positive Control 5:
Rat brain
Predicted Molecular Weight:
Observed: 34kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PTGES2
Additional Names:
C9orf15, GBF-1, GBF1, PGES2, mPGES-2, prostaglandin E synthase 2, GATE-binding factor 1, gamma-interferon-activated transcriptional element-binding factor 1, mPGE synthase-2, membrane-associated prostaglandin E synthase 2, microsomal prostaglandin E synthase-2, prostaglandin-H(2) E-isomerase
Ncbi Gene Id Number:
80142
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-991

List Size:
100 uL

List Price:

$436.00

Datasheet
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