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The purity of rh PTH1R / PTHR1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of rh PTH1R / PTHR1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.

PTHR1 Recombinant Protein

CATALOG NUMBER: 96-641

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Asp 27 - Gly 188
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
19.3 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. When rhPTH1R is used at 2 μg/mL, the concentration of biotinylated human PTHrP that produces 50% of the optimal binding response is found to be approximately 0. 1 - 0. 25 μg/mL.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
PTH1R
Additional Names:
PTH1R, PTHR, PTHR1
Protein Accession Number:
NP_000307
Ncbi Gene Id Number:
5745
Background:
Parathyroid hormone/parathyroid hormone-related peptide receptor is also known as Parathyroid hormone 1 receptor (PTH1R), PTHR1, PTH/PTHrP type I receptor (PTH/PTHr receptor), which belongs to the G-protein coupled receptor 2 family. PTH1R / PTHR1 is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Background Reference 1:
Johnston C.A., et al., 2008, Structure 16:1086-1094.
Background Reference 2:
Pioszak A.A., et al., 2010, J. Biol. Chem. 285:12435-12444.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-641

List Size:
0.1 mg

List Price:

$463.00

Datasheet
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