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42-056 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-056 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PTMA Antibody

CATALOG NUMBER: 42-056

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DVDTKKQKTDEDD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx. 38kDa band in lysates of cell line A431 after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 12.2kDa according to NP_001092755.1 and of 12.1kDa according to NP_002814.3. The 38kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001092755.1; NP_002814.3).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PTMA
Additional Names:
PTMA, prothymosin, alpha, MGC104802, TMSA, gene sequence 28, prothymosin alpha protein, prothymosin, alpha (gene sequence 28), region containing prothymosin, alpha (gene sequence 28), prothymosin, alpha (gene sequence 28), prothymosin, alpha (gene sequence 28), hypothetical protein MGC12250, prothymosin, alpha (gene sequence 28)
Protein Accession Number:
NP_001092755.1,NP_002814.3
Protein Gi Number:
151101407
Ncbi Gene Id Number:
5757
Background Reference 1:
Uren AG, Kool J, Matentzoglu K, de Ridder J, Mattison J, van Uitert M, Lagcher W, Sie D, Tanger E, Cox T, Reinders M, Hubbard TJ, Rogers J, Jonkers J, Wessels L, Adams DJ, van Lohuizen M, Berns A. Large-scale mutagenesis in p19(ARF)- and p53-deficient mice identifies cancer genes and their collaborative networks. Cell 2008 May 133 (4): 727-41.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-056

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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