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Western blot analysis in U251,NCI-H460 cell line lysates (35ug/lane).
PTN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in U251,NCI-H460 cell line lysates (35ug/lane).

PTN Antibody

CATALOG NUMBER: 56-308

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This PTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-154 amino acids from the C-terminal region of human PTN.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTN
Additional Names:
Pleiotrophin, PTN, Heparin-binding brain mitogen, HBBM, Heparin-binding growth factor 8, HBGF-8, Heparin-binding growth-associated molecule, HB-GAM, Heparin-binding neurite outgrowth-promoting factor 1, HBNF-1, Osteoblast-specific factor 1, OSF-1, PTN, HBNF1, NEGF1
Protein Accession Number:
P21246
Protein Gi Number:
131553
Ncbi Gene Id Number:
5764
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PTN ia a heparin binding mitogenic protein. It has neurite extension activity.
Background Reference 1:
Li, F., et al. J. Biol. Chem. 285(36):27673-27685(2010)
Background Reference 2:
Dos Santos, C., et al. Int. J. Cancer 127(5):1038-1051(2010)
Background Reference 3:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 4:
Svensson, S.L., et al. J. Biol. Chem. 285(21):16105-16115(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-308

List Size:
400 ul

List Price:

$495.00

Datasheet
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