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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human prostate carcinoma with PTP4A2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of widr cells using PTP4A2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of PTP4A2 Antibody with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

PTP4A2 Antibody

CATALOG NUMBER: 62-493

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This PTP4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-59 amino acids from the Central region of human PTP4A2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTP4A2
Additional Names:
Protein tyrosine phosphatase type IVA 2, HU-PP-1, OV-1, PTP(CAAXII), Protein-tyrosine phosphatase 4a2, Protein-tyrosine phosphatase of regenerating liver 2, PRL-2, PTP4A2, PRL2, PTPCAAX2
Protein Accession Number:
Q12974
Protein Gi Number:
68566159
Ncbi Gene Id Number:
8073
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PTP4A2 belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity.
Background Reference 1:
Ewing,R.M., et.al., Mol. Syst. Biol. 3, 89 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-493

List Size:
400 ul

List Price:

$495.00

Datasheet
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