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Western blot analysis in K562 cell line lysates (35ug/lane).
PTPN20A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

PTPN20A Antibody

CATALOG NUMBER: 56-046

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PTPN20A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the Central region of human PTPN20A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTPN20A
Additional Names:
Tyrosine-protein phosphatase non-receptor type 20, hPTPN20, PTPN20A
Protein Accession Number:
Q4JDL3
Protein Gi Number:
121944218
Ncbi Gene Id Number:
26095
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene belongs to the family ofclassical tyrosine-specific protein tyrosine phosphatases. Manyprotein tyrosine phosphatases have been shown to regulatefundamental cellular processes and several are mutated in humandiseases. Chromosome 10q contains a segmental duplication resultingin multiple copies of the protein tyrosine phosphatase,non-receptor type 20 gene. The two nearly identical copies aredesignated as PTPN20A and PTPN20B. A third copy is only partiallyduplicated and contains a pseudogene, designated as PTPN20C. Thisgene encodes the more telomeric copy, PTPN20B. Multiplealternatively spliced transcript variants encoding differentisoforms have been identified.
Background Reference 1:
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Background Reference 2:
Fodero-Tavoletti, M.T., et al. Biochem. J. 389 (PT 2), 343-354 (2005) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-046

List Size:
400 ul

List Price:

$495.00

Datasheet
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