Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Immunofluorescent analysis of U251 cells, using PTPN6 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of PTPN6 Antibody in HL-60 cell line lysates (35ug/lane)
Western blot analysis of PTPN6 using rabbit polyclonal PTPN6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2).(2ug/ml)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunofluorescent analysis of U251 cells, using PTPN6 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

PTPN6 Antibody

CATALOG NUMBER: 61-522

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PTPN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of human PTPN6.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:100
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTPN6
Additional Names:
Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, PTP-1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTPN6, HCP, PTP1C
Protein Accession Number:
P29350
Protein Gi Number:
131469
Ncbi Gene Id Number:
5777
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PTPN6 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.
Background Reference 1:
Korporaal,S.J., Arterioscler. Thromb. Vasc. Biol. 29 (3), 372-379 (2009)
Background Reference 2:
Cho,Y.S., Am. J. Respir. Cell Mol. Biol. 39 (4), 412-419 (2008)
Background Reference 3:
Christophi,G.P., Lab. Invest. 88 (3), 243-255 (2008)
Background Reference 4:
Jones,M.L., J. Biol. Chem. 279 (39), 40475-40483 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-522

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369