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46-253 staining (0.3ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
46-253 staining (0.3ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PTPRT Antibody

CATALOG NUMBER: 46-253

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: CNTWEKPMLDQAV
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 164kDa according to NP_573400.2 and 163kDa according to NP_008981.3). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms. Epitope corresponding to aa 58-69 of human PTPRT protein.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PTPRT
Additional Names:
PTPRT, RPTPrho, KIAA0283, protein tyrosine phosphatase, receptor type, T, receptor protein tyrosine phosphatase, RP5-1121H13.2
Protein Accession Number:
NP_573400.2,NP_008981.3
Protein Gi Number:
48762922
Ncbi Gene Id Number:
11122
Background Reference 1:
Wang Z, Shen D, Parsons DW, Bardelli A, Sager J, Szabo S, Ptak J, Silliman N, Peters BA, van der Heijden MS, Parmigiani G, Yan H, Wang TL, Riggins G, Powell SM, Willson JK, Markowitz S, Kinzler KW, Vogelstein B, Velculescu VE. Mutational analysis of the tyrosine phosphatome in colorectal cancers. Science. 2004 May 21;304(5674):1164-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-253

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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