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Antibody used in WB on Mouse embryonic fibroblast at: 1:1000 (Lane 1: 50ug mouse embryonic fibroblast lysate, Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate, Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein).
Antibody used in WB on Mouse NIH-3T3 at 0.625 ug/ml.
Antibody used in IHC on Mouse at of 5 ug/ml.
Antibody used in WB on Mouse embryonic fibroblast at: 1:1000 (Lane 1: 50ug mouse embryonic fibroblast lysate, Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate, Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein).

PTRF Antibody

CATALOG NUMBER: 28-592

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PTRF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PTRF antibody can be used for detection of PTRF by ELISA at 1:312500. PTRF antibody can be used for detection of PTRF by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight:
43 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PTRF antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PTRF
Additional Names:
PTRF, Cavin, Cav-p60, AW546441, 2310075E07Rik
Protein Accession Number:
NP_033012
Protein Gi Number:
6679567
Ncbi Gene Id Number:
19285
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes.
Background Reference 1:
Miyoshi, K., et al., (2001) Genomics 71 (2), 150-155.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-592

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

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