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Antibody used in WB on Human Hela at 0.2-1 ug/ml.
Antibody used in WB on Human 293T at 0.5 ug/ml.
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.
Antibody used in WB on Human Hela at 0.2-1 ug/ml.

PUF60 Antibody

CATALOG NUMBER: 29-418

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PUF60.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PUF60 antibody can be used for detection of PUF60 by ELISA at 1:62500. PUF60 antibody can be used for detection of PUF60 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Tranfected 293T Cell Lysate
Predicted Molecular Weight:
58 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PUF60 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PUF60
Additional Names:
PUF60, FIR, FLJ31379, RoBPI, SIAHBP1, VRJS
Protein Accession Number:
NP_055096
Protein Gi Number:
17298690
Ncbi Gene Id Number:
22827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.
Background Reference 1:
Gao, J., (2008) Genomics 91 (4), 347-355.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-418

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

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