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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

PUF60 Antibody

CATALOG NUMBER: 57-685

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PUF60 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human PUF60.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PUF60
Additional Names:
Poly(U)-binding-splicing factor PUF60, 60 kDa poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, FBP-interacting repressor, Ro-binding protein 1, RoBP1, Siah-binding protein 1, Siah-BP1, PUF60, FIR, ROBPI, SIAHBP1
Protein Accession Number:
Q9UHX1
Protein Gi Number:
74761960
Ncbi Gene Id Number:
22827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a Ro RNP-bindingprotein. It interacts with Ro RNPs and their interaction is thoughtto represent a gain of function for Ro RNPs. This protein alsoforms a ternary complex with far upstream element (FUSE) andFUSE-binding protein. It can repress a c-myc reporter via the FUSE.It is also known to target transcription factor IIH and inhibitactivated transcription. This gene is implicated in the xerodermapigmentosum disorder. There are two alternatively splicedtranscript variants of this gene encoding different isoforms. Thereseems to be evidence of multiple polyadenylation sites for thisgene.
Background Reference 1:
Hsiao, H.H., et al. Biochemistry 49(22):4620-4634(2010)
Background Reference 2:
Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)
Background Reference 3:
Gao, J., et al. Genomics 91(4):347-355(2008)
Background Reference 4:
Hastings, M.L., et al. PLoS ONE(6), E538 (2007) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-685

List Size:
400 ul

List Price:

$495.00

Datasheet
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