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Western Blot at 1:1000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PUM1 using PUM1 Antibody (Y83) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM1 gene (Lane 2).
Formalin-fixed and paraffin-embedded humanbrain tissue reacted with Phospho-PUM1-Y83.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 ug per lane.

PUM1 Antibody

CATALOG NUMBER: 62-999

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Chicken
Immunogen:
This PUM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from human PUM1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
126 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PUM1
Additional Names:
Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, KIAA0099, PUMH1
Protein Accession Number:
Q14671
Protein Gi Number:
41688619
Ncbi Gene Id Number:
9698
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PUM1 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of this protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation.
Background Reference 1:
Gupta,Y.K., Structure 16 (4), 549-557 (2008)
Background Reference 2:
Spassov,D.S.,IUBMB Life 55 (7), 359-366 (2003)
Background Reference 3:
Spassov,D.S.,Gene 299 (1-2), 195-204 (2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-999

List Size:
400 ul

List Price:

$495.00

Datasheet
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