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Western blot analysis of PUM2 using PUM2 Antibody (S182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2).
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PUM2 using PUM2 Antibody (S182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2).

PUM2 Antibody

CATALOG NUMBER: 63-175

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PUM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from human PUM2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
114 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PUM2
Additional Names:
Pumilio homolog 2, Pumilio-2, PUM2, KIAA0235, PUMH2
Protein Accession Number:
Q8TB72
Protein Gi Number:
41688714
Ncbi Gene Id Number:
23369
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
Background Reference 1:
Kusz,K., Mol. Reprod. Dev. 74 (6), 795-799 (2007)
Background Reference 2:
Spik,A., Reprod BiolSUPPL 1, 37-42 (2006)
Background Reference 3:
Moore,F.L., Proc. Natl. Acad. Sci. U.S.A. 100 (2), 538-543 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-175

List Size:
400 ul

List Price:

$495.00

Datasheet
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