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SDS-PAGE analysis of recombinant PUMA fragment on Coomassie Blue-stained 4-20% gradient gel.
SDS-PAGE analysis of recombinant PUMA fragment on Coomassie Blue-stained 4-20% gradient gel.

PUMA Fragment Recombinant Protein

CATALOG NUMBER: 95-102

Tested Applications:
ELISA, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
aa. 76 - 170
Fusion Tag:
Fusion Partner: His-tag and strepII-tag at N-terminus
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Predicted Molecular Weight:
15 kDa (Calculated)
Purity:
~95%
physical-state:
Liquid
Buffer:
1X PBS containing 0.1% SDS and 0.02% NaN3
Storage Conditions:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Ncbi Official Symbol:
BBC3
Additional Names:
PUMA Antibody : JFY1, PUMA, JFY-1
Protein Accession Number:
Q9BXH1
Protein Gi Number:
56748610
Ncbi Gene Id Number:
27113
Background:
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA- and PUMA- (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. This PUMA recombinant protein is recognized by several ProSci PUMA monoclonal antibodies, but not the two polyclonal antibodies (PSI # 3041 and 3043).
Background Reference 1:
Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
Background Reference 2:
Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
Background Reference 3:
Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

95-102

List Size:
0.05 mg

List Price:

$289.00

Datasheet
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