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PURA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in A549,NCI-H292 cell line lysates (35ug/lane).
PURA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

PURA Antibody

CATALOG NUMBER: 56-040

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PURA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the C-terminal region of human PURA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PURA
Additional Names:
Transcriptional activator protein Pur-alpha, Purine-rich single-stranded DNA-binding protein alpha, PURA, PUR1
Protein Accession Number:
Q00577
Protein Gi Number:
1346918
Ncbi Gene Id Number:
5813
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene product is a sequence-specific, single-strandedDNA-binding protein. It binds preferentially to the single strandof the purine-rich element termed PUR, which is present at originsof replication and in gene flanking regions in a variety ofeukaryotes from yeasts through humans. Thus, it is implicated inthe control of both DNA replication and transcription. Deletion ofthis gene has been associated with myelodysplastic syndrome andacute myelogenous leukemia.
Background Reference 1:
Inoue, T., et al. Prostate 69(8):861-873(2009)
Background Reference 2:
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Background Reference 3:
Kaminski, R., et al. Cancer Biol. Ther. 7(12):1926-1935(2008)
Background Reference 4:
Darbinian, N., et al. J. Alzheimers Dis. 15(1):71-82(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-040

List Size:
400 ul

List Price:

$495.00

Datasheet
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