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Purinergic Receptor Antibody

CATALOG NUMBER: 46-883

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-RSEANLQSKSED
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx. 50kDa band in lysates of Human Lung and of cell line A549 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 42.1kDa according to NP_002554.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
P2RY1
Additional Names:
P2RY1, purinergic receptor P2Y, G-protein coupled, 1, P2Y1, ATP receptor, P2 purinoceptor subtype Y1, P2Y purinoceptor 1, platelet ADP receptor, purinergic receptor P2Y1
Protein Accession Number:
NP_002554.1
Protein Gi Number:
4505557
Ncbi Gene Id Number:
5028
Background Reference 1:
Arthur JF, Shen Y, Mu FT, Leon C, Gachet C, Berndt MC, Andrews RK. Calmodulin interacts with the platelet ADP receptor P2Y1. Biochem J. 2006 Sep 15;398(3):339-43.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-883

List Size:
0.1 mg

List Price:

$335.00

Out of stock

Datasheet
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